Biology

Difference between YAC vector and BAC vector

Main difference

The main difference between YAC vector and BAC vector is that YAC vectors are the vector systems contained by yeast within them for the replication of molecular components and can contain data of size 100-1000kb, while BAC vectors are vector systems contained by artificial alterations. Bacterial chromosomal vectors for the replication of molecular components and can contain a size of 100-200 kb.

Vector YAC vs. Vector BAC

YAC vectors contain yeast inside for the replication of molecular components, which means that their DNA is altered to incorporate yeast components, but the BAC vector contains artificially altered bacterial chromosomal vectors for the replication of molecular components.

YAC vectors are formed by using telomeric, centromeric and origin of replication sequences to replicate yeast cells, while BAC vectors are formed by using functional fertility plasmid or plasmid F injecting it into bacteria such as E. coli, transforming or transforming clone it. YAC vectors use yeast cells as hosts to complete the process, while BAC vectors use bacterial cells as host and F plasmid to complete the process.

The basis of YAC vectors is very regional, that is, specific regions of yeast chromosomes can only be used to use it as a vector, while for BAC vectors, plasmid F is used to carry out the properties of the vector. of these bacteria. As far as configuration is concerned, the YAC vectors are configured linearly, but the BAC vectors are configured in a circular region.

The copying capacity is only one per yeast cell in YAC vectors, while in BAC vectors one to two are copied per cell. The cloning capacity is very different since YAC vectors have a higher capacity in terms of data retention, since data of up to 1000 kb can be inserted into these cells, while in BAC vectors the copy capacity is much lower. and data up to 200 kb can only be inserted to perform the whole process.

Comparative chart
Yac vector BAC vector
These vectors contain yeast inside for the replication of molecular components. These contain artificially altered bacterial chromosomal vectors for the replication of molecular components.
Training
Through the use of sequences of telomeric, centromeric and replication origin By using functional fertility plasmid or F plasmid injecting it into bacteria such as E. coli.
Host
Yeast cells Bacterial cells
Region specification
Regionally specified Unspecified.
Setting
Linear Circular
Number of copies
One per yeast cell One or two per bacterial cell
Cloning capacity
High cloning capacity up to 1000 kb Lower cloning capacity up to 200 kb

What is YAC Vector?

YAC vectors are the vector system that uses yeast as vectors for the replication of molecular components, which means that their DNA is altered to incorporate the required component. These are also artificial vector systems for inserting DNA fragments.

Thus, large DNA fragments can be inserted into these vector systems to achieve multiple applications in the preparation of genomic libraries. Thus, after the production of these cells, the required product is obtained.

YAC vectors are formed by using telomeric and centromeric DNA regions in conjunction with origin of replication sequences for replication and cloning of yeast cells. The required trait is copied into these specified regions in yeast cells, which in turn is obtained after the reproduction of these cells.

The elements of the yeast chromosomes used in the process are made up of elements, where CEN is the centromere of the yeast chromosome, which helps in the segregation of the chromosome into two daughter cells in the process of reproduction.

ARS acts as the origins of replication for self-replication in a yeast cell, and TEL is the telomeric region. TRP1 and URA3 act as identifiable marker genes. The bacterial selectable marker gene along with the BamHI and EcoRI restriction are the sites for DNA fragment linearization and data insertion.

YAC vectors use yeast cells as hosts to complete the process. The basis of these vectors is very regional, that is, specific regions of yeast chromosomes can only be used to use it as a vector. As far as configuration is concerned, the YAC vectors are configured linearly, as discussed above.

The copying capacity is only one per yeast cell in YAC vectors. Cloning capacity, on the other hand, varies very differently as YAC vectors have a higher capacity in terms of data retention as data of up to 1000 kb can be inserted into these cells.

What is BAC Vector?

BAC Vector is the type of vector systems that use artificially altered bacterial chromosomal vectors for the replication of molecular components. These are also artificial vector systems for inserting DNA fragments.

Large DNA fragments can be inserted into these vector systems. BAC vectors use bacterial cells as vector systems due to the use of plasmid F in performing the vector process to achieve traits of these bacteria. These vector systems are formed through the use of functional fertility plasmid or plasmid F injecting it into bacteria such as E. coli, transforming or cloning it.

The main components of these BAC vectors are composed of repE that facilitates the mediation of the segregation complex formation, parA and parB are used for gene partitioning during this segregation. The selectable markers, in this case, are used as an antibiotic resistance gene or LacZ, T7 and SP6 are used to facilitate the transcription of the insertion process, the OriS component is used for the unidirectional disruption of replication.

Regarding the configuration, in the case of these vectors, the configuration is carried out in a circular region. The copying capacity in the case of BAC vectors is greater than that of YAC vectors, since one to two are copied per bacterial cell.

The cloning capacity, on the other hand, varies very differently as BAC Vectors, the cloning capacity is much lower, and data up to 200 kb can only be inserted into a singular bacterial cell that performs the whole process.

Key differences

  1. YAC vectors contain yeast inside for the replication of molecular components; on the other hand, BAC Vector contains artificially altered bacterial chromosomal vectors.
  2. YAC vectors use yeast cells as host to complete the process, on the other hand, BAC vectors use bacterial cells as host and plasmid F.
  3. The basis of the YAC vectors are highly regional specified for yeast chromosomes for use as a vector; conversely, for BAC vectors, F plasmids are used to carry out the vector properties of these bacteria.
  4. The YAC vectors are linearly configured; however, the BAC vectors are configured in a circular region.
  5. YAC vectors are formed by using telomeric, centromeric, and origin of replication sequences to replicate yeast cells; on the other hand, BAC vectors are formed by using functional fertility plasmid or F plasmid by injecting it into bacteria such as E. coli by transforming or cloning it.
  6. The copying capacity is only one per yeast cell in YAC vectors; on the other hand, in BAC vectors, one to two are copied per cell.
  7. YAC vectors have a higher capacity in terms of data retention, so they are efficient in terms of colonization, since data of up to 1000 kb can be inserted into these cells, while in BAC vectors the copy capacity is much smaller and only data up to 200 kb can be inserted. to carry out the process.

Final Thought

The YAC vector is housed in yeast for the replication of molecular components and may contain data of size 100-1000 kb, but the BAC vector is contained in artificially altered bacterial chromosomal vectors for the replication of molecular components and may contain the size of 100 -200 kb.

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