Biology

Difference between absorption spectrum and action spectrum

Main difference

The main difference between Absorption Spectrum and Action Spectrum is that Absorption Spectrum shows the absorption of light of different wavelengths by a pigment, while Action Spectrum shows the relative effectiveness of these wavelengths in photosynthesis …

Absorption spectrum v s. Spectrum of action

Plants use light to obtain energy or food through photosynthesis. There are different types of photosynthetic pigments in plants that absorb light of different wavelengths. But they absorb only the light that falls in the region of the visible spectrum, that is, 390 nm to 760 nm. A graphical representation showing the absorption of light of different wavelengths by a plant pigment is known as its absorption spectrum. On the other hand, a graphical representation that shows the effectiveness of these different wavelengths in photosynthesis is known as the action spectrum. An absorption spectrum shows the relationship between the absorption capacity of the pigment and the quality of light. On the other hand, an action spectrum shows the relationship between different wavelengths of light and photosynthetic activity. Chlorophyll absorbs blue and red light, while carotenoids absorb violet and blue light. On the other hand, maximum photosynthesis is carried out with blue and red light.The absorption of light of different wavelengths can be measured using a spectrometer, while in the action spectrum, the rate of photosynthesis can be found out by measuring the amount oxygen production, carbon dioxide fixation and NADP + reduction, etc. According to experiments, the peak of the action spectrum of chlorophyll is the same as that of its absorption spectrum, indicating that chlorophyll acts as the primary pigment in photosynthesis. Chlorophyll absorbs blue and red light, while carotenoids absorb violet and blue light. On the other hand, maximum photosynthesis is carried out with blue and red light.The absorption of light of different wavelengths can be measured using a spectrometer, while in the action spectrum, the rate of photosynthesis can be found out by measuring the amount oxygen production, carbon dioxide fixation and NADP + reduction, etc. According to experiments, the peak of the action spectrum of chlorophyll is the same as that of its absorption spectrum, indicating that chlorophyll acts as the primary pigment in photosynthesis. Chlorophyll absorbs blue and red light, while carotenoids absorb violet and blue light. On the other hand, maximum photosynthesis is carried out with blue and red light.The absorption of light of different wavelengths can be measured using a spectrometer, while in the action spectrum, the rate of photosynthesis can be found out by measuring the amount oxygen production, carbon dioxide fixation and NADP + reduction, etc. According to experiments, the peak of the action spectrum of chlorophyll is the same as that of its absorption spectrum, indicating that chlorophyll acts as the primary pigment in photosynthesis. The absorption of light of different wavelengths can be measured using a spectrometer, while in the action spectrum, the rate of photosynthesis can be found out by measuring the amount of oxygen production, carbon dioxide fixation and reduction of NADP +, etc. According to experiments, the peak of the action spectrum of chlorophyll is the same as that of its absorption spectrum, indicating that chlorophyll acts as the primary pigment in photosynthesis. The absorption of light of different wavelengths can be measured using a spectrometer, while, in the action spectrum, the rate of photosynthesis can be found out by measuring the amount of oxygen production, carbon dioxide fixation and reduction of NADP +, etc. According to experiments, the peak of the action spectrum of chlorophyll is the same as that of its absorption spectrum, indicating that chlorophyll acts as the primary pigment in photosynthesis.

Comparative chart
Absorption spectrum Spectrum of action
A graphical representation showing the absorption of light of different wavelengths by a plant pigment is known as the absorption spectrum. A graphical representation that shows the effectiveness of different wavelengths of light in photosynthesis is known as an action spectrum.
It represents
Represents the intensity of the absorbed light in relation to its specific wavelength. It shows the relative efficiency of photosynthesis produced through light of different wavelengths.
Explain
An absorption spectrum explains the relationship between the absorption capacity of the pigment and the quality of light. An action spectrum shows the relationship between different wavelengths of light and photosynthetic activity.
Measurement
The absorption of light of different wavelengths can be measured using the spectrometer. In the action spectrum, the photosynthesis rate can be found out by measuring the amount of oxygen production, carbon dioxide fixation, and NADP + reduction, etc.
Examples
Chlorophyll absorbs blue and red light, but carotenoids absorb violet and blue light. Maximum photosynthesis takes place in blue and red light.

What is the absorption spectrum ?

An absorption spectrum is a graph that represents light of different wavelengths absorbed by different pigments in plants. A solution of a pigment is placed in a device known as a spectrometer that gives the wavelengths that are absorbed by the pigment. The light absorbed by the given pigment is plotted against the wavelength given by its absorption spectrum. Explain the relationship between the absorption capacity of the pigment and the quality of light. Represents the intensity of the absorbed light in relation to its specific wavelength. According to the absorption spectrum, chlorophyll absorbs blue and red colored light, but carotenoids absorb violet and blue colored light.

What is Action Spectrum ?

An action spectrum is a graph that represents the effectiveness of different wavelengths of light absorbed by different pigments in photosynthesis. The rate of photosynthesis in an action spectrum can be found out by measuring the amount of oxygen production, carbon dioxide fixation, and NADP + reduction, etc. Explain the relationship between different wavelengths of light and photosynthetic activity. It shows the relative efficiency of photosynthesis produced through light of different wavelengths. Depending on the spectrum of action of the different pigments in plants, maximum photosynthesis occurs in blue and red light.

Key differences

  1. A graphical representation showing the absorption of light of different wavelengths by a plant pigment is known as an absorption spectrum, while a graphical representation showing the effectiveness of different wavelengths of light in photosynthesis is known as a spectrum. of action.
  2. An absorption spectrum represents the intensity of absorbed light in relation to its specific wavelength; on the other hand, an action spectrum shows the relative efficiency of photosynthesis produced through light of different wavelengths.
  3. An absorption spectrum explains the relationship between the absorption capacity of the pigment and the quality of light in reverse; an action spectrum shows the relationship between different wavelengths of light and photosynthetic activity.
  4. The absorption of light of different wavelengths can be measured using a spectrometer on the other side, in the action spectrum, the photosynthesis rate can be found out by measuring the amount of oxygen production, carbon dioxide fixation and reduction of NADP +, etc.
  5. The absorption spectrum of different pigments shows that chlorophyll absorbs blue and red light but, carotenoids absorb violet and blue light, on the other hand, the action spectrum shows that maximum photosynthesis takes place in blue light. and red.
Final Thought

The above discussion summarizes that the absorption spectrum and the action spectrum play an important role in determining the rate of photosynthesis. An absorption spectrum is the graphic representation of the absorption of light by different pigments; on the other hand, the action spectrum is the graphic representation that represents the action of different wavelengths of light in photosynthesis.

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